TABLE 3

Effects of heterologous expression of metallo-β-lactamase-encoding genes from E. tenax strain Stok-2 (blaESP-1), C. piscium strain Stok-1 (blaCPS-1), and P. roseus strain SI-33 (blaPEDO-1) on MICs of β-lactams in E. coli TOP10

Antimicrobial agentEUCAST clinical breakpoint (μg/ml)bMIC (μg/ml)
E. coliE. coli pESP-1E. coli pCPS-1E. coli pPEDO-1
Meropenema>80.0320.50.0940.094
Ceftazidime>4≤0.2532416
Cefazolin≤8>16≤816
Cefepime>4≤1≤1≤1≤1
Cefoxitin8>646464
Cefpodoxime>10.53242
Cefotaxime>2<0.2520.50.5
Ceftriaxone>2≤12≤1≤1
Ampicillin>8≤42566464
Piperacillin-tazobactam>16≤416≤4≤4
Ceftazidime-clavulanic acid≤0.251648
Cefotaxime-clavulanic acid0.1220.250.5
  • a MIC was determined by Etest.

  • b −, data could not be obtained.