TABLE 5

Mitochondrial protein synthesis inhibitiona

CompoundMean EC50 (μM) ± SD
COX1SDHACell viability
Compound 12>15039.5 ± 9.223.0 ± 1.4
Compound 13>15020.5 ± 2.180.0 ± 5.7
Compound 14>15021.5 ± 6.4106 ± 37.5
Linezolid27.3 ± 10.8>150>150
Chloramphenicol31.4 ± 23.2>150110 ± 14.1
Doxycycline23.7 ± 6.4109 ± 29118 ± 35.5
Erythromycin>150>150>150
  • a COX1 is cytochrome c oxidase, which is a mitochondrial protein that is synthesized by mitochondrial ribosomes. SDHA is subunit A of the succinate dehydrogenase complex, which is a mitochondrial protein that is synthesized by cytoplasmic ribosomes. Janus green staining was used to determine cell viability after 7 days.