TABLE 1

Plasmids and bacterial strains used in this study

Plasmid or strainDescription or genotypeReference or source
Plasmids
    pMS81ϕBT1 attP-int-derived integration vector for conjugal transfer of DNA from E. coli to Streptomyces spp. (Hygr)35
    pIJ10257pMS81 carrying ermEp with ribosome binding site and multicloning sites (Hygr)26
    pIJ6902Integrative (ϕC31 attP-int) and conjugative (oriT RK2) tipAp expression vector (Aprr Thior)30
    pHJH2000pMS81 carrying vanRSst including the van promoter sequenceThis study
    pHJH2002pGEM-T Easy carrying vanRst including the van promoter sequenceThis study
    pHJH2003pMS81 carrying vanRst including the van promoter sequenceThis study
    pHJH2005pGEM-T Easy carrying vanSstThis study
    pDU006pIJ10257 carrying vanSst under the control of ermEpThis study
    pHJH2006pIJ6902 carrying vanSst under the control of ermEpThis study
    pJG001pIJ10257 carrying vanRst under the control of ermEpThis study
    pGN078pIJ10257 carrying vanRst(L10P, M12L) under the control of ermEpThis study
    pGN083pIJ10257 carrying vanRst(V62I) under the control of ermEpThis study
    pGN082pIJ10257 carrying vanRst(T69S) under the control of ermEpThis study
    pGN080pIJ10257 carrying vanRst(Q92G) under the control of ermEpThis study
    pGN081pIJ10257 carrying vanRst (R106Q, I109A, I112L) under the control of ermEpThis study
    pGN079pIJ10257 carrying vanRst(L10P, M12L, R106Q, I109A, I112L) under the control of ermEpThis study
    pGN073pMS81 carrying vanRSsc including the van promoter sequenceThis study
    pHJH2001pIJ10257 carrying vanRsc under the control of ermEpThis study
    pGN067pMS81 carrying vanRsc including the van promoter sequenceThis study
    pHJH2004pGEM-T Easy carrying vanSscThis study
    pDU001pIJ10257 carrying vanSsc under the control of ermEpThis study
Strains
    M600S. coelicolor SCP1 SCP233
    H27S. coelicolor M600(pMS81)31
    J3201S. coelicolor ΔvanRSsc SCP1 SCP227
    H21S. coelicolor ΔvanRsc SCP1 SCP2This study
    J3200S. coelicolor ΔvanSsc SCP1 SCP227
    H28S. coelicolor J3201(pMS81)This study
    H2474S. coelicolor H21(pMS81)This study
    H2473S. coelicolor J3200(pMS81)This study
    H2060S. coelicolor J3201(pHJH2000)This study
    H2123S. coelicolor H21(pHJH2003)This study
    H36S. coelicolor J3200(pDU006)This study
    H2297S. coelicolor J3201(pGM067)This study
    H2062S. coelicolor J3201(pHJH2003)This study
    H2255S. coelicolor H21(pGN078)This study
    H2259S. coelicolor H21(pGN083)This study
    H2328S. coelicolor H21(pGN082)This study
    H2263S. coelicolor H21(pGN080)This study
    H2500S. coelicolor H21(pGN081)This study
    H2504S. coelicolor H21(pGN079)This study
    H2257S. coelicolor J3201(pGN078)This study
    H2261S. coelicolor J3201(pGN083)This study
    H2329S. coelicolor J3201(pGN082)This study
    H2265S. coelicolor J3201(pGN080)This study
    H2501S. coelicolor J3201(pGN081)This study
    H2505S. coelicolor J3201(pGN079)This study
    NRRL 15009S. toyocaensis A47934 producer, wild type16
    H2251S. toyocaensis NRRL 15009(pMS81)This study
    H31S. toyocaensis ΔvanRSst::aprThis study
    H320S. toyocaensis ΔvanRSst::apr(pHJH2006)This study
    H33S. toyocaensis ΔvanSst::aprThis study
    H229S. toyocaensis H31(pMS81)This study
    H2205S. toyocaensis H320(pMS81)This study
    H2118S. toyocaensis H33(pMS81)This study
    H2360S. toyocaensis H31(pGN073)This study
    H2309S. toyocaensis H320(pGN067)This study
    H312S. toyocaensis H33(pDU001)This study
    H2476S. toyocaensis H31(pHJH2003)This study
    H2311S. toyocaensis H31(pGN067)This study
    H2233S. toyocaensis H320(pGN078)This study
    H2239S. toyocaensis H320(pGN083)This study
    H2336S. toyocaensis H320(pGN082)This study
    H2245S. toyocaensis H320(pGN080)This study
    H2502S. toyocaensis H320(pGN081)This study
    H2506S. toyocaensis H320(pGN079)This study
    H2231S. toyocaensis H31(pGN078)This study
    H2237S. toyocaensis H31(pGN083)This study
    H2338S. toyocaensis H31(pGN082)This study
    H2243S. toyocaensis H31(pGN080)This study
    H2503S. toyocaensis H31(pGN081)This study
    H2507S. toyocaensis H31(pGN079)This study
    ET12567(pUZ8002)E. coli ET12567 containing helper plasmid pUZ800233
    BW25113(pIJ790)E. coli BW25113 containing helper plasmid pIJ79036
    BT340E. coli DH5α containing pCP20 (temperature-sensitive FLP recombination plasmid)36