TABLE 1

Composition and coding parameters of WT and CpG/UpA-low luciferase sequences

SequenceSymbolNo. of substitutionsa% C+G contentNo. of CpG dinucleotidesΔCpGbRatio of O/E frequencies for CpGcNo. of UpA dinucleotidesΔUpAbRatio of O/E frequencies for UpACAIdCPSe
WTL0.441001.21860.700.719−0.011
CpG/UpA-lowl1740.450100022640.170.8040.021
  • a Number of sequence changes from the WT sequence.

  • b Changes in the numbers of CpG and UpA dinucleotides.

  • c Ratio of observed to expected frequencies of CpG and UpA dinucleotides.

  • d Calculated by using the CAIcal website (http://genomes.urv.es/CAIcal/) (40).

  • e Codon pair score; calculated as previously described (26, 41).