TABLE 1

Antibacterial activity of piscidin-1 and its analogs against different bacteria in the absence and presence of 10% serum and their therapeutic indices

PeptideMIC (μM)HC50a (μM)GMb MIC (μM)Therapeutic indexc
E. coli ATCC 25922S. aureus ATCC 25923P. aeruginosa ATCC BAA-427K. pneumoniae ATCC 27736B. subtilis ATCC 6633In serumOf labeled peptide
E. coli ATCC 25922S. aureus ATCC 25923E. coli ATCC 25922S. aureus ATCC 25923
Piscidin-13.0 ± 0.41.25 ± 0.26.0 ± 0.83.8 ± 0.68.0 ± 1.115 ± 2.012 ± 2.03.5 ± 0.51.5 ± 0.5153.684.07
I5A-piscidin-110.0 ± 1.45.0 ± 0.720.0 ± 2.512.0 ± 1.616.0 ± 2.0>50>5012 ± 2.06.0 ± 1.050011.3943.89
I9A-piscidin-15.0 ± 0.72.5 ± 0.410.0 ± 1.46.0 ± 0.88.0 ± 1.120 ± 2.518 ± 2.06.0 ± 1.03.0 ± 0.51505.6926.36
I16A-piscidin-16.0 ± 0.82.5 ± 0.412.0 ± 1.68.0 ± 1.18.0 ± 1.120 ± 2.518 ± 2.06.5 ± 1.03.0 ± 0.55006.4977.04
I5V-piscidin-15.0 ± 0.72.5 ± 0.412.0 ± 1.66.0 ± 0.88.0 ± 1.120 ± 2.518 ± 2.05.5 ± 0.83.0 ± 0.5405.906.77
I9V-piscidin-15.0 ± 0.72.0 ± 0.412.0 ± 1.66.0 ± 0.88.0 ± 1.120 ± 2.518 ± 2.05.5 ± 0.82.4 ± 0.4355.656.19
I16V-piscidin-15.0 ± 0.72.0 ± 0.412.0 ± 1.66.0 ± 0.88.0 ± 1.120 ± 2.518 ± 2.05.5 ± 0.82.4 ± 0.4405.657.07
I5F,F6A-piscidin-110.0 ± 1.45.0 ± 0.724.0 ± 3.012.0 ± 1.616.0 ± 2.0>50>5012 ± 2.06.0 ± 1.015011.8112.70
V12I-piscidin-12.0 ± 0.41.0 ± 0.24.0 ± 0.63.0 ± 0.46.0 ± 0.812 ± 2.010 ± 1.42.5 ± 0.41.0 ± 0.272.702.59
  • a HC50, mean concentration of peptide producing 50% hemolysis.

  • b GM, geometric mean.

  • c The therapeutic index is HC50/geometric mean MIC.