TABLE 2

Kinetic parameters of recombinant PvHPPK-DHPS

PvHPPK-DHPSkcat (s−1)Km pABA (μM)Ki (μM)
SulfadoxineSulfathiazoleDapsone
Wild type0.0024 ± 0.00030.15 ± 0.021.50 ± 0.110.15 ± 0.050.11 ± 0.01
A383G mutant0.0055 ± 0.00050.33 ± 0.0248.10 ± 5.452.95 ± 0.113.67 ± 0.60
A383G A553G mutant0.0134 ± 0.00050.48 ± 0.05176.19 ± 8.7015.53 ± 1.374.54 ± 0.51
S382A A383G A553G mutant0.0060 ± 0.00080.26 ± 0.01266.83 ± 16.3119.00 ± 1.7417.73 ± 1.14
V585A mutant0.0029 ± 0.00020.19 ± 0.013.01 ± 0.360.19 ± 0.020.53 ± 0.06