TABLE 1

Quantification of cell death markersa

Sample no.ConstructMiltefosine concn (μM)SbIII concn (μM)% cellsb:
Annexin V, PIAnnexin V+, PIAnnexin V+, PI+Annexin V, PI+
1pCLN099.0010
2ARM58099001
3pCLN8050.427.117.84.76
4ARM588036.8033.3026.703.16
5pCLN09900.600
6ARM5809900.600
7pCLN40089.54.62.43.4
8ARM5840097.30.61.21.0
9pCLN80075.95.78.210.2
10ARM5880090.66.31.61.5
  • a L. donovani promastigotes with pCLN or ARM58 transgenes were challenged with 80 μM miltefosine or 400 to 800 μM SbIII for 72 h. Cells were stained with the cell death markers annexin V and propidium iodide (PI), and 10,000 events were analyzed by fluorescence-assisted cell sorting. Raw data can be found in Fig. S1 in the supplemental material.

  • b Annexin V-negative (annexin V) and PI-negative (PI) cells are viable, annexin V-positive (annexin V+) and PI-negative cells are in early PCD, annexin V-positive and PI-positive (PI+) cells are in PCD, and annexin V-negative and PI-positive cells are ruptured.