TABLE 2

Complementation of E. faecalis 4205 ΔefrCD with a plasmid producing wild-type EfrCD or the inactive E512Q mutant from the native promoter

E. faecalis 4205 genotypeComplementationMIC (μg/ml)a of:
DaunorubicinHoechst 33342
ΔefrCDEfrCD322
EfrCD_E512Q20.25
Wild typeEfrCD322
EfrCD_E512Q161
  • a MICs were determined in cation-supplemented Mueller-Hinton broth with 10 μg/ml erythromycin after 18 h at 37°C from a minimum of three independent experiments.