TABLE 2

EC50, CC50, and SI of artemisone, artesunate, and ganciclovir in different cell culturesd

DrugCell typeEC50 (μM)aCC50 (μM)bSI
ArtemisoneHFF0.68 ± 0.28>164.83 ± 1.53c>242.40c
MRC-50.22 ± 0.0918.67 ± 7.184.86
ARPE-190.49 ± 0.21>121.46 ± 5.83c>247.88c
ArtesunateHFF19.31 ± 2.0453.56 ± 7.052.77
MRC-51.53 ± 0.4214.92 ± 3.19.75
ARPE-197.46 ± 2.1454.49 ± 7.817.30
GanciclovirHFF0.63 ± 0.39>640>1,015.87
MRC-51.67 ± 0.96>640>383.23
ARPE-192.08 ± 0.19>640>307.70
  • a EC50s for HCMV strain TB40/E were determined by the plaque reduction assay. The results represent those from at least 3 independent experiments and are expressed as means ± standard deviations.

  • b CC50 values (the drug concentration resulting in a 50% reduction of cell viability) were determined by the MTT assay. The results represent those from at least 3 independent experiments and are expressed as means ± standard deviations.

  • c As shown, in HFF and ARPE cells, the DMSO-only control demonstrated cellular toxicity. The four CC50 and SI values indicated relate to the artemisone concentrations for which the respective concentrations of the DMSO-only control demonstrated a 50% reduction of cell viability, thus limiting the ability to evaluate the effect of higher artemisone concentrations. The CC50 and SI of artemisone are therefore indicated to be greater than the value indicated for these cells.

  • d HFF, human foreskin fibroblasts; MRC-5, human fetal lung fibroblasts; ARPE, human retinal pigmented epithelial cells; SI, selectivity index. Artemisone was stored as a 10 mM stock solution in DMSO, and the respective concentrations of the DMSO-only control were tested in parallel in all assays.