TABLE 1

Comparison of olorofim MIC determined using plates prepared using serial dilution or ISO dilution and by adopting visual full inhibition or spectrophotometer endpoint criteria

Isolate (n)MIC (mg/liter)
Serial dilution platesISO plates
Full inhibitionSpectrophotometric inhibition (%)Full inhibitionSpectrophotometric inhibition (%)
Reader 1Reader 2–4a95908550Reader 1Reader 2–4a95908550
A. flavus (10)
    Median0.030.030.030.0160.0160.0010.030.030.0160.0160.0160.002
    Range0.030.030.016–0.030.016–0.030.008–0.030.0005–0.0080.3–0.1250.016–0.060.016–0.030.008–0.0160.004–0.0160.00024–0.008
    No. 2-fold dilutions112235332236
A. fumigatus WT (5)
    Median0.060.030.030.030.0160.0160.030.030.030.0160.0160.008
    Range0.060.016–0.060.030.016–0.030.016–0.030.004–0.0160.03–0.060.016–0.060.016–0.030.016–0.030.008–0.0160.001–0.008
    No. 2-fold dilutions131223232224
A. fumigatus mutant (5)
    Median0.060.030.030.030.030.0040.060.030.030.030.030.004
    Range0.06–0.1250.03–0.1250.03–0.1250.016–0.030.016–0.030.004–0.030.03–0.060.03–0.060.03 to >10.016–0.060.016–0.060.004–0.03
    No. 2-fold dilutions233224227334
A. nidulans (10)
    Median0.060.060.030.030.030.0080.060.060.030.030.0160.004
    Range0.03–0.1250.03–0.1250.03–0.060.016–0.030.016–0.030.004–0.0160.03–0.1250.3–0.1250.016–0.060.016–0.030.016–0.030.002–0.016
    No. 2-fold dilutions332223333224
A. niger (10)
    Median0.060.060.50.060.030.0160.060.060.50.060.030.008
    Range0.03–0.250.03–0.1250.03 to >10.016 to >10.016–0.250.008–0.0320.03–0.1250.03–0.250.06 to >10.016 to >10.016–0.250.002–0.03
    No. 2-fold dilutions437853346854
A. terreus (10)
    Median0.030.0160.0160.0160.0080.0040.030.0160.0080.0080.0080.002
    Range0.016–0.060.008–0.1250.008–0.030.008–0.0160.004–0.0160.0005–0.0080.008–0.060.008–0.030.004–0.0160.004–0.0080.004–0.0080.00024–0.004
    No. 2-fold dilutions353235433225
  • Species-specific MIC ranges broader than the optimal target (spanning ≤3 dilutions) are underlined.

  • a Reader 1 was constant throughout the study, whereas the second reader was any of 3 different individuals (Reader 2 to 4).