TABLE 4

Expression levels of select genes following CA or CST adaptation

StrainFold upregulationaMutationFold increase in MIC over WT
phoPphoQsmvAsmvRpmrKpmrDpagPCHDCST
M109 CA10ΔsmvR C terminus (CT)4None
NCTC 13439 CA19123ΔsmvR16>32
M3 CA15918764197PhoP E82K, ΔsmvR8>32
NCTC 13443 CA552762662PhoQ A20P, ΔsmvR CT16>32
NCTC 13368 CA87186PhoP Y98C16>32
MGH 78578 CA68246PhoQ L348Q16>32
M109 WT pACYC phoQ A20Pb3724phoQ A20P on plasmidNone64
KP16 CST542057PhoQ T244NNone>64
M3 CST853874PhoQ L348QNone>64
51851 vs 4670410960116MgrB Q30STOPNone>64
NCTC 13438 CST55PmrB D150YNone>64
  • a With respect to wild-type levels, unless otherwise indicated.

  • b Significant fold upregulation compared to WT or pACYC phoPQ WT.