Table 3.

Outcross and incross of flqA(grlB543) locus by transformation with chromosomal DNA

DonorRecipientMIC (μg/ml) for recipient:Phenotype of transformantaNo. of trans- formants
StrainGenotypeStrainGenotypeCiprofloxacinNovobiocinCiprofloxacinNovobiocin
ISP2133 trp-489Ω(chr::Tn917lac)2 MT5224c9 nov(gyrB142)grlB543 1.0b 5.0c RS137
SR109
EN3 grlB543Ω(chr::Tn917lac)2 MT5 nov(gyrB142)0.25c 40.0b SR92
RS57
  • a S, susceptible; R, resistant. The phenotype was determined by plating each colony on ciprofloxacin at 0.125, 0.5, and 1.0 μg/ml and on novobiocin at 1.25 and 20 μg/ml. For scoring transformants for ciprofloxacin susceptibility, R was defined as growth at 0.125 and 0.5 μg/ml and no growth at 1.0 μg/ml and S was defined as growth at 0.125 μg/ml and no growth at 0.5 and 1.0 μg/ml. For scoring transformants for novobiocin susceptibility, R was defined as growth at 1.25 and 20 μg/ml and S was defined as growth at 1.25 μg/ml and no growth at 20 μg/ml.

  • b Value indicates resistance.

  • c Value indicates susceptibility.