Table 4.

Kinetic parameters of TEM-52, TEM-3, TEM-52H, and TEM-1

SubstrateTEM-52TEM-3TEM-52HTEM-1
Km (μM)Ki (μM)aRelative Vmax (%)Km (μM)Ki (μM)Relative Vmax (%)Km (μM)RelativeVmax (%)Km (μM)Relative Vmax (%)
Benzylpenicillin5.3b 1003.81002910026100
Ampicillin4.1668.21264011340122
Cephaloridine13130191205329524425
CAZ23950350659820.2
CTX30229352521,1702.3
MOX0.161.9
  • a Ki constants were measured with cephaloridine used as the substrate.

  • b —, not determined.