Table 4.

Susceptibility patterns of S. aureus RN4220 strains diploid for grlB + and mutant grlB

Strain/plasmidType ofgrlBa in:MIC (μg/ml)b of:
ChromosomePlasmidCPFXNFLXOFLXSPFXNAOANOVCOU
RN4220/pSK950WildNone0.51.00.50.0664.02.00.160.01
RN4220/pBFC9BWildN470D1.08.01.00.1232.02.00.040.0025
EN22/pSK950N470DNone1.08.01.00.1264.02.00.040.0025
EN22/pBFISBN470DWild0.250.50.250.0664.02.00.080.01
  • a Wild, wild-type gene; none, plasmid vector only; N470D, mutant gene (grlB543) encoding Asn470Asp mutation.

  • b CPFX, ciprofloxacin; NFLX; norfloxacin; OFLX, ofloxacin; SPFX, sparfloxacin; NA, nalidixic acid; OA, oxolinic acid; NOV, novobiocin; COU, coumermycin. The MICs are the means of three different transformants.