Table 2.

Multiple-sequence alignment for the PBPs and β-lactamases in the regions of the primary structure critical for catalysisa

SourceCatalytic serine locus Ib ∗∗Ser-130 or Tyr-150 locus IIc ∗∗Lys-234, Lys-315, or His-298 locus IIId ∗∗AeBfCgDhEi
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  • a The numbering system for the sequences is according to the sequence for each protein as deposited in the GenBank or SWISS-PROT data banks.

  • b Asterisks represent the position for the catalytic serine and the lysine residue three residues away (analogous to the Lys-73 of class A β-lactamases).

  • c Asterisks represent the position for Ser-130 and Asn-132 of class A β-lactamases and the analogous positions in the related proteins.

  • d Asterisks represent the position for Lys-234 of class A β-lactamases and the analogous positions in the related proteins.

  • e A, the sequence number for the catalytic serine marked with an asterisk in the second column.

  • f B, the sequence number for the serine or tyrosine residue marked with an asterisk in the third column.

  • g C, The sequence number for the lysine and histidine residues marked with an asterisk in the fourth column.

  • h D, the distance (number of amino acids) between loci in the third and second columns.

  • i E, the distance (number of amino acids) between loci in the fourth and third columns.