Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or characteristicaSource or reference
Strains
S. aureus
  ISP7948325pig-131 33
  ISP21338325 pig-131 trp-489Ω(chr::Tn917lac)2 37
  MT58325 nov (gyrB142) hisG15 pig-131 37
  MT5224c98325nov (gyrB142) hisG15 pig131 flqA(grlB543) 37
  EN38325nov (gyrB142) hisG15 pig-131 flqA(grlB543) Ω(chr::Tn917lac)2 25
  RN42208325-4 r 17
  EN5RN4220 gyrB142 flqC(gyrA) 25
  EN8RN4220flqA (grlA542) Ω(chr::Tn917lac)2 nov(gyrB142) flqC(gyrA) 25
  EN14RN4220flqA (grlB543) Ω(chr::Tn917lac)2 nov(gyrB142) flqC(gyrA) 25
  EN20RN4220flqA (grlA542) Ω(chr::Tn917lac)2 25
  EN22RN4220 flqA (grlA543) Ω(chr::Tn917lac)2 25
  BF2RN4220 nov (gyrB142)This studyb
 E. coliDH5αF-φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17 (rK mK ) supE44λ thi-1 gyrA96 relA1 GIBCO-BRL
Plasmids
 pSK95010.5-kb plasmid carrying the att site of phage L54a; replicon of pSC101, Spr (E. coli) and temperature-sensitive replicon of pE194, Tcr (S. aureus)G. L. Archer
 pGEM3-zf(+)2.9-kb cloning vector, Apr Promega
 pYL112Δ197-kb plasmid carrying the integrase gene of phage L54a; Apr (E. coli); Cmr (S. aureus) 18
 pBFISB2.2-kb BamHI fragment of PCR product containing the grlB gene from ISP794 cloned into pSK950This study
 pBFC9B2.2-kb BamHI fragment of PCR product containing the grlB gene from MT5224c9 cloned into pSK950This study
  • a Ap, ampicillin; Cm, chloramphenicol; Sp, spectionmycin; Tc, tetracycline.

  • b Cross between DNA of MT5 and RN4220.