Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant charactersaSource or reference
K. pneumoniae
 NEM865Tic Caz Mox Cm Sm Gm Su Tmp TcClinical isolate
 NEM533NalSpontaneous mutant of K. pneumoniae 5214-K (28) and this work
 NEM835Nal Tic Caz MoxConjugation NEM865 × NEM533
E. coli
 K802NNal;hsdR + hsdM + gal met supE 33
 TG1F′ traD36 lacIqΔ(lacz)M15proA + B /supEΔ(hsdM-mcrB)5(rk mk McrB)thi Δ(lac-proAB) 11
 NEM837Nal Tic Caz MoxConjugation NEM865 × K802N
Plasmids
 pNEC865Tic Caz Mox; Mob (13.5 kb)This work
 pBGS18Km (cloning vector) 31
 pBGS18Ωbla TEM-52 Km Tic Caz Mox; recombinant derivative of pBGS18 carrying a 1-kbEcoRI-BamHI fragment amplified from pNEC865This work
 pBGS18Ωbla TEM-3 Km Tic Caz; recombinant derivative of pBGS18 carrying a 1-kbEcoRI-BamHI fragment amplified from pCFF04This work
 pBGS18Ωbla TEM-1 Km Tic; recombinant derivative of pBGS18 carrying a 1-kb EcoRI-BamHI fragment amplified from pBR322This work
 pBGS18Ωbla TEM-52-H Km Tic; recombinant derivative of pBGS18 carrying the 660-bpEcoRI-PstI fragment of pBGS18Ωbla TEM-52 and the 340-bpPstI fragment of pBGS18Ωbla TEM-1 This work
  • a Resistance to antibiotics is denoted as follows: Tic, ticarcillin; Caz, ceftazidime; Mox, moxalactam; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Nal, nalidix acid; Su, sulfamide; Tmp, trimethoprim; and Tc, tetracycline. Mob, mobilizable.