Table 2.

Oligonucleotide primers used in PCR and sequencing

PrimerSequence (5′ to 3′)aGene used for primer designPosition inermTRPrimer used with primer
III1 GAA ATT GG(A/C) ACA GGT AAA GGG CA ermAM 316–338III2 b
III2 b AAA (C/T)TG ATT TTT AGT AAA ermAM 828–846III1
III6 GAA GTT TAG CTT TCC TAA ermTR 468–485III2 b
III7 TGC TGT TAA TGG TGG AAA ermTR 641–658III2 b
III8 b GCA TGA CAT AAA CCT TCA ermTR 393–410III9
III9 ACA TAA GGA GGT TTC AAT ermA 1–18III8 b
III10 AGG TTA TAA TGA AAC AGA ermTR 204–221III8 b
III13 b TTA GTG AAA CAA TTT GTA ermA 925–942III7
III14 TCT CCT TGC CGG TTA TAA ermTR 186–203III15 b
III15 ATC AAT TAA GAC AGG TGC TGA AGC ermTR 839–862III14 b
  • a Nucleotides in parentheses indicate that during synthesis either of the nucleotides was incorporated into the oligonucleotide.

  • b The primer was biotinylated at the 5′ end for sequencing purposes.