Table 1.

Plasmids and E. coli strains used in this study

Plasmid or strainGenotypeReference or source
Plasmids
 pDML501pBR325 with a 4.5-kbNcoI insert from E. hirae S185R TcrApr Cmrpbp3r28
 pDML508pBR325 with a 14-kb NcoI insert fromE. hirae S185R Tcr AprCmrerm pbp3r28
 pDML510pBR322 with a 4.5-kb EcoRI insert from E. hirae S185R Tcr Aprpbp3r28
 pDML540pBR322 with a 7-kb EcoRI insert fromE. hirae R40 Tcr Aprpbp59
 pUCBM20AprBoehringer (Mannheim, Germany)
 pGEM3-Zf(+)AprPromega (Leiden, The Netherlands)
E. coli strains
 JM109endA1 recA1 gyrA96 thi hsdR17(rK, mK+) relA1 supE44 λ Δ(lac-proAB) (F′traD36 proAB lacIqZΔM15)Promega (Leiden, The Netherlands)
 DH5αF φ80dlacZΔM15 Δ(lacZYA-argF) U169 dcoR recA1 endA1 phoA hsdR17 (rK, mK+)supE44 λ thi-1 gyrA96 relA1Life Technologies (Gibco BRL, Merelbeke, Belgium)
 RR1Δ(gpt-protA)62 leuB6 thi-1 lacY1 hsdSB20 rpsL20(Strr) ara-14 galK12 xyl-5, mtl-1 supE44 mcrBBUnited States Biochemicals (Cleveland, Ohio)