Table 1.

Clinical data, RAPD patterns, IEF of β-lactamases, and identified β-lactamases of isolates of the familyEnterobacteriaceae and transconjugants

StrainSpeciesDate of isolation (day.mo.yr)Type of specimenHospital, wardType of infectionRAPD patternIsoelectric points of β-lactamasesβ-Lactamase identified by sequence analysis
L-267K. pneumoniae18.1.1995Throat swabŁódź, pediatricPneumonia, pharyngitisA6.0,a7.6TEM-47
L-372K. pneumoniae26.1.1995UrineRzeszów, pediatricUTIbB<5.4, 6.0, 7.6TEM-48
L-380K. pneumoniae1.2.1995UrineRzeszów, pediatricUTIB<5.4, 6.0, 7.6TEM-48
L-491K. pneumoniae15.2.1995UrineRzeszów, pediatricUTIB<5.4, 6.0, 7.6TEM-48
L-577K. pneumoniae23.2.1995UrineRzeszów, surgicalUTIB<5.4, 6.0, 7.6TEM-48
L-439K. pneumoniae4.2.1995UrineRzeszów, pediatricUTIC<5.4, 7.6, 8.2SHV-5-like
L-414K. pneumoniae24.1.1995Tracheostomy tubeŁódź, ICUcSuspected pneumoniaD<5.4, 7.6, 8.2SHV-5-like
L-251K. pneumoniae9.1.1995Bronchial exudateŁódź, pediatricPneumoniaE5.4, 7.6,8.2SHV-5-like
L-95K. pneumoniae4.1.1995Wound swabWarsaw, neurosurgicalWound infectionF7.6, 8.2
L-352K. pneumoniae22.1.1995Tracheostomy tubeŁódź, ICUSuspected pneumoniaG7.6, 8.2
L-902K. pneumoniae7.3.1995Tracheostomy tubeŁódź, ICUSuspected pneumoniaH7.6, 8.2
L-867E. coli30.4.1995Tracheostomy tubeŁódź, ICUSuspected pneumonia5.4, 6.0, >9.0TEM-49
  • a Underlined pI values are those for β-lactamases which were also produced by transconjugants.

  • b UTI, urinary tract infection.

  • c ICU, intensive care unit.