TABLE 1.

Primers used in the study to amplify and sequence the different loci, amplicon sizes, annealing temperatures, and amplicon positions on the respective genes

Gene (accession no.)PrimerSequenceAnnealing temp (°C)Position (nt)Amplicon size (bp)
rpoB (L27989)ForwardGGG AGC GGA TGA CCA CCC602266350
ReverseGCG GTA CGG CGT TTC GAT GAA C2615
katG (X68081)ForwardGCC CGA GCA ACA CCC603237
ReverseATG TCC CGC GTC AGG239
ForwardCGA GGA ATT GGC CGA CGA GTT551187414
ReverseCGG CGC CGC GGA GTT GAA TGA1600
inhA regulator sequenceForwardCCT CGC TGC CCA GAA AGG GA45Upstream of inhA gene248
ReverseATC CCC CGG TTT CCT CCG GT
gyrA (L27512)ForwardCAG CTA CAT CGA CTA TGC GA452383320
ReverseGGG CTT CGG TGT TAC CTC AT2702
gyrB (L27512)ForwardCCA CCG ACA TCG GTG GAT T551538428
ReverseCTG CCA CTT GAG TTT GTA CA1965
rpsl (X70995)ForwardGGC CGA CAA ACA GAA CGT545′ noncoding region505
ReverseGTT CAC CAA CTG GGT GACS7 gene
rrs (Z83862)ForwardTTG GCC ATG CTC TTG ATG CCC541411140
ReverseTGC ACA CAG GCC ACA AGG GA1280