Table 4.

Effect of pbp4 inactivation on vancomycin susceptibility

StrainVancomycin MIC (μg/ml)a
Broth microdilutionE-test
RN450M22
RN450MΔPBP423
RN450MΔPBP4/pJF3b 22
N31511
N315ΔPBP413
N315ΔPBP4/pJF3b 11
COL23
COLΔPBP44.06
COLΔPBP4/pJF3b 2.03
  • a MICs were determined by the broth microdilution and E-test methods as described in Materials and Methods.

  • b MIC determination was done in the presence of chloramphenicol (5 μg/ml) to maintain pressure on pJF3.