Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeReference or source
V. choleraeO139
 MO10Toxigenic 1992 clinical isolate from India, SXTMO10+, SurTmr Cmr Smr 41
 AS207Toxigenic 1997 isolate from Calcutta, SurTmS Cmr Smr 36
 E712Nontoxigenic 1994 isolate from Sri Lanka, SurTmr Cmr Smr 30
 20551998 clinical isolate from Bangladesh, SuSTmS CmS SmS 21
 HKO139-SXTS Clinical isolate from Hong Kong, Sus TmS CmS Sms 47
V. cholerae O1
 CO943El Tor 1994 clinical isolate from India, Sur TmrCmr Smr 44
 1811/98El Tor 1998 clinical isolate from Bangladesh, Sur TmrCmr Smr 21
C10488 El Tor 1999 clinical isolate from Bangladesh, Sur TmrCmr Smr This study
E. coliK-12
 TOP10F′ mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 Invitrogen
 DH5αF thi-1 ΔlacU196φ80lacZΔM15 hsdR17 recA1 endA1
Plasmids
 pSXT1pSuperCos1 containing a part of the SXT element encoding Cmr SurTmr Smr 44
 pWKS30AprpSC101 derivative45
 pATMP1pWKS30 + 14 kbp from SXTMO10, Cmr TmrSmr This study
 pSUL1pWKS30 + 1.7 kbp from SXTMO10, Sur This study
 pYL1pWKS30 + 2.77 kbp from SXTET,dfrA1 + This study
 pYL8pWKS30 + 3.8 kbp from SXTET, dfrA1 + This study
 pGB2Spcr pSC101 derivative6
 pRLH422pGB2 + 11 kbp from R391, Knr 19, this study