Bacterial strains and plasmids used in this study
| Strain, isolate, or plasmid (origin) | Relevant characteristics | Source or reference |
|---|---|---|
| Strains or isolates | ||
| HIP 5836 (New Jersey) | Vancomycin MIC of 8 μg/ml | 23 |
| HIP 6297 (New York) | Vancomycin MIC of 6 μg/ml | 23 |
| HIP 5827 (Michigan) | Vancomycin MIC of 8 μg/ml | 23 |
| Mu50 (Japan) | Vancomycin MIC of 6 μg/ml | 11 |
| Mu3 (Japan) | Vancomycin MIC of 2 μg/ml | 11 |
| RN4220 | Restriction-deficient derivative of 8325-4 | |
| RN450M | Heterogeneous Oxar | 14 |
| N315 | Heterogeneous Oxar | 14 |
| COL | Homogeneous Oxar | 14 |
| 27619 | Homogeneous Oxar | 2 |
| RN450MVR | Passage-derived mutant of RN450M; vancomycin MIC of 8 μg/ml | This study |
| N135VR | Passage-derived mutant of N315; vancomycin MIC of 8 μg/ml | This study |
| COLVR | Passage-derived mutant of COL; vancomycin MIC of 8 μg/ml | This study |
| Plasmids | ||
| pJF3 | 1.8-kb pbp4 gene and 5′ sequences with pRN5543 staphylococcal replicon; Chlr | This study |
| pJF14 | pE194(ts)-based vector with pbp4 interrupted by tetM; ErmrTetr | This study |