Table 1.

Bacterial strains and plasmids used in this study

Strain, isolate, or plasmid (origin)Relevant characteristicsSource or reference
Strains or isolates
 HIP 5836 (New Jersey)Vancomycin MIC of 8 μg/ml23
 HIP 6297 (New York)Vancomycin MIC of 6 μg/ml23
 HIP 5827 (Michigan)Vancomycin MIC of 8 μg/ml23
 Mu50 (Japan)Vancomycin MIC of 6 μg/ml11
 Mu3 (Japan)Vancomycin MIC of 2 μg/ml11
 RN4220Restriction-deficient derivative of 8325-4
 RN450MHeterogeneous Oxar 14
 N315Heterogeneous Oxar 14
 COLHomogeneous Oxar 14
 27619Homogeneous Oxar 2
 RN450MVRPassage-derived mutant of RN450M; vancomycin MIC of 8 μg/mlThis study
 N135VRPassage-derived mutant of N315; vancomycin MIC of 8 μg/mlThis study
 COLVRPassage-derived mutant of COL; vancomycin MIC of 8 μg/mlThis study
Plasmids
 pJF31.8-kb pbp4 gene and 5′ sequences with pRN5543 staphylococcal replicon; Chlr This study
 pJF14pE194(ts)-based vector with pbp4 interrupted by tetM; ErmrTetr This study