TABLE 2.

Calculated Z′ values as a measure of reproducibility of the assays with various strains

StrainZaDynamic rangeb
OD determinationLuminescence assayOD determinationLuminescence assay
Growth platesCV assay with pinsCV assay with platesBlankMutant controlGrowth platesCV assay with pinsCV assay with plates
BlankMutant controlBlankMutant controlBlankMutant control
PA140.68<0.10.670.600.570.370.890.820.164 (0.007)0.268 (0.016)0.967 (0.061)992,640 (36,261)
ZK2870<0.10.530.800.770.770.700.920.890.085 (0.006)0.503 (0.024)1.543 (0.063)2,006,927 (52,801)
PAO10.60<0.10.770.750.470.320.860.820.127 (0.005)0.330 (0.013)0.920 (0.057)1,715,298 (80,165)
PAO1 ΔfliC0.68NA<0.1NA<0.1NA0.87NA0.165 (0.007)0.110 (0.005)0.471 (0.043)225,330 (9,187)
PAK0.60<0.10.270.27<0.1<0.10.690.640.173 (0.007)0.138 (0.008)0.269 (0.024)436,591 (40,870)
PAK ΔfliC0.56NA<0.1NA<0.1NA<0.1NA0.158 (0.006)0.085 (0.005)0.225 (0.023)26,611 (8,569)
BlankNANANANANANANANA0.057 (0.004)0.091 (0.005)0.319 (0.031)156 (77)
  • a The mutant control used for the Z′ calculations for strains PA14, ZK2870, and PAO1 was PAO1 ΔfliC, whereas the mutant control used for the Z′ calculation for strain PAK was PAK ΔfliC. NA, calculation is not applicable.

  • b The dynamic range data consist of raw values observed and demonstrate that luminescence had a range of 12,865 times over that for the blank control, whereas the dynamic range for the OD was a maximum of 5.5 times over that for the blank control. Standard deviations are indicated in parentheses.