TABLE 3.

MICs of different antimicrobials for various strains of P. aeruginosa grown in LB medium

StrainAlleleaMutationMIC (μg/ml)b of:
CIPNORNALIMIGM
Sensitive strains
    H103Wild type0.10.325612
    H110431_C9lon::lux0.0250.1512812
    H110574_D9lon::lux0.0250.07512812
    H110624_A2lon::lux0.0250.1512812
    H110969_C12recA::lux0.050.07525612
    H11216_G1PA4961::lux0.050.07512812
    H112453_F11PA5279::lux0.050.1512812
    H112516_G12recG::lux0.0250.1512812
    H1105/lon+clon::lux/lon+0.10.32561d
Resistant strains
    H103Wild type0.10.325612
    H112970_H3rnt::lux0.4>0.651212
    H113363_H5nfxB::lux0.8>0.651212
    H113675_F11PA5455::lux0.4>0.651212
  • a Mutant alleles in our Tn5-lux library are described online at http://pseudomutant.pseudomonas.com/ and in reference 30.

  • b Results are modes from four independent experiments. CIP, ciprofloxacin; NOR, norfloxacin; NAL, nalidixic acid; IMI, imipenem; GM, gentamicin.

  • c H1105 (lon::lux) complemented with the Lon-expressing plasmid pBBR1-MCS5::lon+ (33).

  • d Not measurable, because this resistance marker was on the cloning plasmid.