TABLE 1.

Mutants selected in 8325-4 as FA resistant and then screened for SCV phenotypes

Strain and stockIdentified mutationaMIC (μg/ml) of:Doubling time (min)bAuxotrophycnd
fusA (EF-G)rplF (L6)FASTRKAN
8325-4NoneNone0.12531.524
FusA classic
    AH207Thr385AsnNone412329
    AH194Pro404LeuNone482252
    AH196Pro406LeuNone68331
    AH198Gly452SerNone3264382
    AH201Gly452CysNone128628
    AH197Leu456PheNone48634
    AH187His457TyrNone9663299
    AH206Arg464SerNone68637
    AH190Arg464HisNone684313
FusA-SCV
    AH188Gly664SerNone4162446Hemin2
FusE
    AH193NoneTAA stop at nt 229424854Hemin
    AH199NoneTAA stop at nt 241416857Hemin
    AH202NoneCTG start at nt 1416858Hemin
    AH204NoneΔ of nt 203-231424857Hemin
    AH209NoneΔ of nt 404-427416860Hemin
  • a None, wild type. Underlined letters represent the mutated residues.

  • b Doubling time (generation time) in minutes during exponential growth in LB.

  • c Auxotrophy tested for hemin, menadione, thymine, and thymidine. −, nonauxotrophic.

  • d Number of independent isolates with the same genotype and phenotype.