TABLE 3

MICs of TAC and its related analogues D6, D15, and D17 against recombinant M. abscessus strains

StrainaMIC (μg/ml) forb:
D6D15D17TAC
CIP104536 S256.225>200
    pMV261_ethAMAB6.251.656.2550
    pMV261_ethAMTB12.53.112.5100
    pMV261_ethRMAB1002550>200
    pMV261_ethRMTB5012.525>200
CIP104536 R12.53.16.2100–200
    pMV261_ethAMAB3.11.61.650
    pMV261_ethAMTB3.11.63.150
    pMV261_ethRMAB5012.550>200
    pMV261_ethRMTB5012.525>200
  • a M. abscessus was transformed with different constructs to overexpress either the EthAMAB (MAB_0985) or EthRMAB (MAB_0984c) protein from M. abscessus or from M. tuberculosis, as indicated.

  • b Data are representative of three independent experiments. The MIC was determined in cation-adjusted Mueller-Hinton broth.