Table 2.

Nucleotide sequences of the oligonucleotides used forbla TEM andbla OXA-1 amplification

Gene and primerSequencePositiona
bla TEM
 A5′-ATAAAATTCTTGAAGAC-3′−7
 B5′-AAAACTCTCAAGGATCTT-3′382
 C5′-AAAGATGCTGAAGATCA-3′301
 D5′-TTTGGTATGGCTTCATTC-3′726
 E5′-TTACCAATGCTTAATCA-3′652
 F5′-TTTTTTGCACAACATGGG-3′1069
bla OXA-1
 G5′-TCAACTTTCAAGATCGCA-3′211
 H5′-GTGTGTTTAGAATGGTGA-3′820
  • a For the positions of thebla TEM gene primers the numbering of Sutcliffe (34) was used. For the positions of thebla OXA-1 gene primers the numbering of Ouellette et al. (26) was used.