Table 5.

Nucleotide substitutions determined by sequencing of the previously oligotyped IRT-encoding genes and thebla TEM gene of strain AP7 compared with the sequence of bla TEM-1A

Nucleotide positionaNucleotide change (amino acid)b
blaTEM-1AblaTEM-33blaTEM-34blaTEM-36blaTEM-37blaTEM-38blaTEM-39AP7
32CT
162GTTTT
175AGGG
226CT
346AGGGG
436CTTTTTT
604GT
682TCCC
925GA
929C (Arg-244)A (Ser)
980G (Val-261)A (Ile)
  • a Nucleotide numbering is according to Sutcliffe (34).

  • b The amino acid is indicated when a point mutation leads to an amino acid substitution compared with the sequence of TEM-1 (pBR322). The numbering is according to Ambler et al. (2).