Table 1.

Bacterial strains and plasmids used

E. colistrain or plasmidCharacteristic(s)Reference or source
Strains
 TUM1083Clinical isolate, cefotaxime-resistant strainThis study
 TUM1103Transformant, E. coli AS226 harboring pMTY036This study
 ML4909F galK2 galT22 hsdR metB1 relA supE44 Rifr M. Inoue
 C600F thi-1 thr-2 leuB6 lacY1 tonA21 supE44 λ Rifr 27
 AS226F thr-1 leuB6 thi-1 hsdS1 lacY1 tonA21 supE44 ampCΔ λ 13
 MV1184 ara Δ (lac-proAB) Δ (srl-racA) 306::Tn10 φ80dlacZ ΔM15 rpsL thi [F′lacI q lacZ ΔM15proAB traD36] 31
Plasmids
 pHSG397 lac cat 30
 pMTY002Cefotaxime resistantThis study
 pMTY0362.1-kb Sau3AI fragment from pMTY002 cloned into pHSG397This study
 R388IncW 33
 R386IncFI 27
 R100-1IncFII 27
 R124IncFIV 27
 R64-11IncIα 27
 R621αIncIγ 10
 R27IncHI 27
 N3IncN 27
 R751IncP 15
 R446BIncM 27
 R401IncT 27
 R6KIncX 27