Table 1.

Mutations within the grlA, grlB,gyrA, and gyrB genes in 116 clonally unrelated clinical isolates of S. aureus from eight countries

Gene and base position(s) (change[s])aAmino acid changeNo. of strains
Ciprofloxacin susceptibleCiprofloxacin resistantMSSAMRSA
grlA
 2495 (A→C)*None2020
 2614 (C→T)Ser-80→Phe160160
 2651 (C→T)*None3030
 2756 (A→G)*None1001
 2614 (A→C); 2626 (A→T)*Ser-80→Phe; Glu-84→Val0404
 2614 (A→C); 2751 (T→C)*Ser-80→Phe; none0101
 2517 (G→A); 2614 (C→T)*Ala-48→Thr; Ser-80→Phe0413
 2510 (T→G); 2516 (T→C); 2606 (T→C)*Ile-45→Met; none; none2020
 2714 (G→A); 2798 (T→C)*; 2805 (C→T); 2825 (A→G)*None; none; Pro-144→Ser; none4022
 2510 (T→G); 2516 (T→C)*; 2606 (T→C); 2614 (C→T)*Ile-45→Met; none; none; Ser-80→Phe1010
 2497 (T→G); 2510 (T→G); 2516 (T→C)*; 2606 (T→C); 2614 (C→T)*Val-41→Gly; Ile-45→Met; none; none; Ser-80→Phe0110
 No mutations3201022
  Total46702393
grlB
 1644 (C→T)*None1010
 1650 (A→T)*Glu-422→Asp1010
 1677 (T→C)*None1607
 1679 (A→G)*Asp-432→Gly0101
 1735 (C→T)*Pro-451→Ser0101
 1866 (T→C)*None1001
 1650 (A→T); 1776 (A→G); 1866 (T→C)*Glu-422→Asp; none; none1010
 1644 (C→T); 1650 (A→T); 1710 (A→T)*; 1770 (A→T); 1866 (T→C)*None; Glu-422→Asp; none; none; none3140
 1644 (C→T); 1647 (T→A); 1650 (A→T)*; 1776 (A→G); 1866 (T→C)*None; none; Glu-422→Asp; none; none3021
 No mutations35611482
  Total46702393
gyrA
 2402 (C→T)Ser-84→Leu043142
 2409 (T→C)None160313
 2413 (G→A)Glu-88→Lys1010
 2481 (T→C)None2130
 2487 (A→G)Ser-112→Arg1010
 2402 (C→T); 2409 (T→C)Ser-84→Leu; none0817
 2402 (C→T); 2481 (T→C)*Ser-84→Leu; none0110
 2409 (T→C); 2413 (G→A)*None; Glu-88→Lys016016
 2402 (C→T); 2409 (T→C); 2468 (G→A)*Ser-84→Leu; none; Gly-106→Asp0101
 No mutations2601214
  Total46702393
gyrB
 1534 (G→A)None2020
 1579 (A→G)*None1626735
 1525 (C→T); 1579 (A→G)*None; none1001
 1474 (T→A); 1498 (C→T)*None; none1010
 No mutations26441357
  Total46702393
  • a *, new single point mutations or combinations of single point mutations.