Table 2.

Expression of an oprM-lacZ fusion in MexAB OprM+ and MexAB OprMP. aeruginosaa

StrainPlasmidβ-Galactosidase activity (Miller units)b
PAO6609pMP19016.2 ± 2.9
PAO6609pRSP4383.2 ± 6.0
PAO6609pRSP4412.4 ± 1.5
K1032pMP19017.4 ± 3.1
K1032pRSP4373.4 ± 3.6
K1032pRSP446.9 ± 0.5
  • a P. aeruginosa PAO6609 (MexAB OprM+) and K1032 (ΔmexAB oprM) carrying pMP190 or the oprM-lacZ pMP190 derivatives pRSP43 and pRSP44 were cultured in LB broth with appropriate antibiotic to anA600 of 1.0 and assayed for β-galactosidase activity as described in Materials and Methods.

  • b Values are means of two separate experiments performed in triplicate ± standard deviations.