Table 1.

Oligonucleotides used in this study

DesignationSequence (5′ to 3′)aPositionsbStrandcPurposedReference or source
DP1ACGGCGGCCGTAACTATA1985–2002+Commonly used for SDM, sequencing 30
ZGE23ACAGGCCAGTTAGCTA2292–2277Commonly used for SDM, sequencing 30
GW1GACGGGAAGACCCCGTGGA2137–2155+SDM (A2142G)This work
GW11GGTCTTCCCGTCTTGCCGC2148–2130SDM (A2142G)This work
GW2GACGGCAAGACCCCGTGGA2137–2155+SDM (A2142C)This work
GW12GGTCTTGCCGTCTTGCCGC2148–2130SDM (A2142C)This work
GW3GACGGTAAGACCCCGTGGA2137–2155+SDM (A2142T)This work
GW13GGTCTTACCGTCTTGCCGC2148–2130SDM (A2142T)This work
GW4GACGGAGAGACCCCGTGGA2137–2155+SDM (A2143G)This work
GW14GGTCTCTCCGTCTTGCCGC2148–2130SDM (A2143G)This work
GW5GACGGACAGACCCCGTGGA2137–2155+SDM (A2143C)This work
GW15GGTCTGTCCGTCTTGCCGC2148–2130SDM (A2143C)This work
GW6GACGGATAGACCCCGTGGA2137–2155+SDM (A2143T)This work
GW16GGTCTATCCGTCTTGCCGC2148–2130SDM (A2143T)This work
GW7AGACGAAAAGACCCCGTGG2136–2154+SDM (G2141A)This work
GW17GTCTTTTCGTCTTGCCGCG2147–2129SDM (G2141A)This work
  • a The bases different from those in the wild-type 23S rRNA gene sequence are underlined.

  • b Positions refer to the new numbering scheme for the H. pylori 23S rRNA (30).

  • c The plus (+) strand is equivalent to the rRNA sequence, and the minus (−) strand is the complementary one.

  • d SDM, site-directed mutagenesis.