Table 1.

Bacterial strains used in this study

StrainOrganismPlasmidCharacteristicspI of β-lactamase(s)Source or reference
28 S. typhimurium pCLL3425Clinical isolate8.8This study
34 S. typhimurium pCLL3422 + pCLL3425Clinical isolate7.6, 8.8This study
DH5α E. coli E. coli K-12 cloning strainNone 14
F φ80dlacZΔM15 Δ(lacZYA-argF) U169recAI endAI hsdR17 (rKmK+) supE44 thiI gyrI relAI
DH5α E. coli pCLL2300Kmr cloning vectorNone 24
DH5α E. coli pCLL3422 + pCLL2300Transconjugant of S. typhimurium 347.6This study
DH5α E. coli pCLL3425Transformant of S. typhimurium348.8This study
DH5α E. coli pCLL34177-kbBamHI fragment containing bla CTX-M-5in pCLL23008.8This study