Table 1.

Bacterial strains used in this study

StrainOrganismPlasmidCharacteristicspI of β-lactamase(s)Source or reference
28S. typhimuriumpCLL3425Clinical isolate8.8This study
34S. typhimuriumpCLL3422 + pCLL3425Clinical isolate7.6, 8.8This study
DH5αE. coliE. coli K-12 cloning strainNone14
F φ80dlacZΔM15 Δ(lacZYA-argF) U169recAI endAI hsdR17 (rKmK+) supE44 thiI gyrI relAI
DH5αE. colipCLL2300Kmr cloning vectorNone24
DH5αE. colipCLL3422 + pCLL2300Transconjugant of S. typhimurium 347.6This study
DH5αE. colipCLL3425Transformant of S. typhimurium348.8This study
DH5αE. colipCLL34177-kbBamHI fragment containing blaCTX-M-5in pCLL23008.8This study