Table 1.

S. aureus strains and plasmids used in this study

Strain or plasmidGenotype or characteristicaSource or reference
Strains
 ISP21338325 pig-131 trp-489Ω(chr::Tn917lac)2 14
 MT5224c48325 pig-131 nov (gyrB142)hisG15 flqA (grlA542) 14
 MT5224c28325 pig-131 nov (gyrB142)hisG15 flqA (grlA552) 11
 MT1118325 pig-131 hisG15 flqA(grlA548) 14
 EN18325pig-131 nov (gyrB142) hisG15 flqA(grlA542) Ω(chr::Tn917lac)2 11
 BF38325 pig-131 hisG15 flqA (grlA548) Ω(chr::Tn917lac)2 This study: ISP2133 DNA × MT111
 BF48325 pig-131 nov(gyrB142) hisG15 flqA (grlA552) Ω(chr::Tn917lac)2 This study: ISP2133 DNA × MT5224c2
 RN42208325-4 r 9
 EN20RN4220flqA (grlA542) Ω(chr::Tn917lac)2 11
 BF5RN4220 flqA (grlA548) Ω(chr::Tn917lac)2 This study: BF3 DNA × RN4220
 BF6RN4220 flqA (grlA552) Ω(chr::Tn917lac)2 This study: BF4 DNA × RN4220
 BF2RN4220 nov(gyrB142) 7
 BF7RN4220nov (gyrB142) flqA(grlA552) Ω(chr::Tn917lac)2 This study: BF4 DNA × BF2
 BF8RN4220 nov (gyrB142)flqA (grlA548) Ω(chr::Tn917lac)2 This study: BF3 DNA × BF2
 BF9RN4220 nov (gyrB142)flqA (grlA542) Ω(chr::Tn917lac)2 This study: EN1 DNA × BF2
Plasmids
 pSK95010.5-kb plasmid carrying the attP site of phage L54a, replicon of pSC101, Spr (E. coli) and temperature-sensitive replicon of pE194, Tcr (S. aureus)G. L. Archer
 pSKISA2.7-kb BamHI fragment containing thegrlA gene from ISP794 cloned into pSK950This study
 pSKMTA2.7-kb BamHI fragment containing thegrlA gene from MT111 cloned into pSK950This study
 pSKC2A2.7-kb BamHI fragment containing thegrlA gene from MT5224c2 cloned into pSK950This study
 pSKC4A2.7-kb BamHI fragment containing thegrlA gene from MT5224c4 cloned into pSK950This study
 pBFISA100-bp EcoRI fragment containing the promoter of grlB cloned into pSKISAThis study
 pBFMTA100-bp EcoRI fragment containing the promoter of grlB cloned into pSKMTAThis study
 pBFC2A100-bp EcoRI fragment containing the promoter of grlB cloned into pSKC2AThis study
 pBFC4A100-bp EcoRI fragment containing the promoter of grlB cloned into pSKC4AThis study
  • a Sp, spectinomycin, Tc, tetracycline.