Table 1.

Properties of resistant clinical isolates of S. pneumoniae

Isolate no.Serotype/cloneaAntibiogrambPCR resultSize(s) (kb) of AvaI fragment(s) in Tn917c
ermAMtet(M)aphA-3
E123F/SpanishPN, CM, TC, EM, CC++2.3, 1.60, 1.27
E223F/SpanishPN, CM, TC, EM, CC++2.3, 1.75, 1.27
E166B/Spain-IcelandPN, CM, TC, EM, CC++2.3, 1.75, 1.27
1003NontypeablePN, TC, EM, CC++2.3, 1.75, 1.27
E41NontypeablePN, TC, EM, CC++2.3, 1.75, 1.27
BM4200NontypeablePN, CM, TC, EM, CC+++22.5
E623F/SpanishPN, CM, TC, EM, CC++16
E126B/Spain-IcelandPN, CM, TC, EM, CC++22
E4219APN, EM, CC++22
E436B/Spain-IcelandPN, TC, EM, CC++19
35436B/Spain-IcelandPN, CM, TC, EM, CC++17.5
26496B/Spain-IcelandPN, CM, TC, EM, CC++22
  • a Clonal type relatedness was determined by PFGE.

  • b The antibiogram indicates resistance to the indicated drugs. The resistance breakpoints were as follows: penicillin (PN), MIC ≥ 2 μg/ml; chloramphenicol (CM), MIC ≥ 8 μg/ml; tetracycline (TC), MIC ≥ 8 μg/ml; erythromycin (EM), MIC ≥ 1 μg/ml; clindamycin (CC), MIC ≥ 1 μg/ml.

  • c Molecular size(s) of AvaI-digested genomic DNA fragments hybridized with digoxigenin-labeled Tn917.