Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristicsaSource or reference
Strains
S. enteritidisKF92Clinical strain producing DHA-1 17
E. coli HB101F l hsdS20 recA13 ara-14 proA2 leu lacY1 galK2 rpsL20 xyl-55 mtl-1 supE44 10
E. coliJM101 supE thi Δ(lac-proAB) (F′ tra D36 proAB lacI q ZΔM15)Messing, 1979
E. coli J53-2 (J62.6)Transconjugant producing BIL-1; Tcr Nalr 40
E. coli C600 (pMG231)Transformant producing MIR-1; Cmr 39
Plasmids
 pACYC184Cloning vector; CmrTcr 13
 pNH5 HpaI fragment containing ampD gene from E. cloacae transferred into pBGS18 Kmr M. H. Nicolas
 pSAL1Conjugative plasmid of 95 kb encoding DHA-1 17
 Clone pSAL2-indRecombinant plasmid; 4.9-kb Sau3A-Sau3A fragment encoding DHA-1This study
  • a Cmr, chloramphenicol resistant; Nalr, nalidixic acid resistant; Tcr, tetracycline resistant; Kmr, kanamycin resistant.