Table 3.

Comparison of total cytochrome P-450 content and fluconazole susceptibility of 14α-demethylases of C. albicans and C. krusei a

OrganismFluconazole IC50 (μM)bCytochrome P-450 content (nmol/mg of protein)
C. albicansY01.3450.017 ± 0.0050.077 ± 0.037
C. albicansSC53140.032 ± 0.0060.058 ± 0.037
C. krusei91-11580.751 ± 0.3840.052 ± 0.021
C. krusei 91-11590.495 ± 0.2080.065 ± 0.018
C. krusei91-11610.795 ± 0.4360.077 ± 0.060
  • a Results are the means ± standard deviations of at least three separate measurements.

  • b Concentration of fluconazole required to inhibit the synthesis of ergosterol from [14C]mevalonic acid by 50%.