Table 2.

Specific binding of [3H]sordarin to macromolecules in cell extracts from wild-type and GM193663r mutant strainsa

StrainMutationdpmb
Expt 1Expt 2
373None85,64071,265
 FPR1-3Ala562Pro1250
 FPR1-5Phe612Leu1720
SEY6210None23,184188,515
 FPR1-18Ile529Ser22976
 FPR1-19Ser523Thr0191
 FPR1-21Tyr521Asp087
 FPR1-24Val561Phe4,8531,040
 FPR1-26Ala475Val01,770
 FPR1-30Pro559Ser997,053
  • a Resistant isolates are listed below their corresponding parental strain.

  • b 3H radioactivity associated with macromolecules, measured in two independent experiments (experiments 1 and 2), as described in Materials and Methods.