Table 1.

Effect of AY on HIV-1 production and cell mortality in PBMCs from patients with AIDSa

Time (days)DonorProduction of HIV-1 in culture supernatants% Mortality of cultured cells
p24 protein (pg/ml)HIV-1 particles (counts/ml of medium)
AY−AY+AY−AY+AY−AY+
10M154900700239
14M1109800770288
22M170065,1708222610
10M2−126507251810
14M2359216805511
22M22,500257,5001,0007012
10M31071,1006521612
14M31,8002,2002,5413,1611312
22M315106508113013
10M4547501,000187
14M47101,0001,4002510
22M42001501,3001,0002813
10M5544005002510
14M51067006413211
22M58−25004003512
10M6−13600400199
14M621500520298
22M6434203804013
10M7476195422013
14M7655316002712
22M7−124505503114
10M8−136005752511
14M8245004503514
22M8434805304216
  • a After activation with PHA, PBMCs were cultured for up to 25 to 30 days in complete medium with recombinant IL-2 in the presence (AY+) or in the absence (AY−) of the molecule. The cells were harvested for cell viability determination, and the culture medium was replaced every 3 days in order to maintain a concentration of 8 × 105 cells/ml. The culture supernatants were used to test both HIV-1 p24 antigen levels by enzyme-linked immunosorbent assay and reverse transcriptase activity. Mortality was assessed by blue Trypan blue coloration. Data are expressed as the means for triplicate cultures. The standard deviation was less than 10% of the mean.