Table 1.

Ammonia and glutamate generated from 2 mM glutamine by Caco-2/TC-7 cellsa

ProductAmt (nmol/h/106viable cells) produced under the following conditions:
ControlControl + ofloxacinInfectionInfection + ofloxacin
Ammonia218.17 ± 15.02209.87 ± 12.14254.23 ± 18.85b 240.31 ± 10.25
Glutamate88.16 ± 20.7885.44 ± 21.14102.0 ± 16.3692.06 ± 7.34
  • a Uninfected (control) and infected (with C53) Caco-2/TC-7 cells were incubated for 1 h with glutamine and without or with ofloxacin. The net generation of ammonia and glutamate was measured. Values are means ± standard deviations for five to eight experiments.

  • b The P value (in a comparison with the control) was <0.001.