Table 5.

Binding of OPC-20011 and reference compounds to PBPs in E. coli NIHJ JC-2

CompoundIC50 (μg/ml)a
PBP1APBP1BPBP2PBP3PBP4PBP5PBP6
OPC-20011>62.52.56<0.1<0.1>62.5>62.518.84
OPC-200261.521.571.74<0.1>62.5>62.5>62.5
FMOX0.101.26>62.5<0.11.220.140.14
CPR10.132.784.04<0.138.43>62.5>62.5
  • a Binding affinities of test compounds for PBPs were expressed as the concentration (in micrograms per milliliter) required to prevent 50% of the binding of [14C]benzylpenicillin to each PBP.