Table 1.

Effect of linezolid on IF2-tRNAfMet binary complex formationa

SampleConcn (μM)dpm obtained with IF2 at the following concn:
0.5 pmol5 pmol
Control085110,426
Linezolid20076512,980
  • a Duplicate reaction mixtures containing either 0.5 or 5 pmol of purified E. coli IF2 and 10,000 dpm of [35S]tRNAfMet were incubated for 10 min at 37°C and were trapped on Millipore filters as described in Materials and Methods.