Table 1.

Strains and plasmids

Strain or plasmidGenotype or descriptionResistance phenotypeaReference or source
Strain
 PAe191Clinical strain ofP. aeruginosa Ti Ca Ak Gm Su HgThis work
 DH5α E. coli; F′/endA1 hsdR17 (rk mk +)supE44 thi-1 recA1 gyrA (Nalr) relA1 D(lacIZYA-argF)U169 deoR[f80 dlacD(lacZ)M15]NalNew England Biolabs
 PAO38RifRifampin-resistant derivative of P. aeruginosa PAO38Ri (MIC, >500 μg/ml) 20
Plasmid
 pHSS62.3-kb plasmid vectorKm 32
 pK182.7-kb plasmid vectorKm 25
 pNJR3-228-kb E. coli-P. aeruginosa shuttle vectorTc 28
 pAZ30523-kbEcoRI fragment obtained from PAe191 total DNA, cloned into pHSS6; oxa19 Km Ti Ca Gmb This work
 pAZ3164.0-kb HindIII fragment from pAZ305 cloned into HindIII-digested pNJR3-2;oxa19 Tc Ti Ca Gmb This work
 pAZ3271,097-bp NarI-HindIII fragment from pAZ316 cloned intoAccI-HindIII-digested pK18,oxa19 Km Ti CaThis work
 pAZ309pNJR3-2 derivative encoding oxa13 Tc Ti Ak 20
 pAZ310In vitro-selected derivative of pAZ309 selected on ceftazidime; oxa13-1 Tc Ti Ca AkThis work
 pAZ3261,097-bpNarI-HindIII fragment from pAZ310 cloned intoAccI-HindIII-digested pK18;oxa13-1 Km Ti CaThis work
  • a Ti, ticarcilline; Ca, ceftazidime; Km, kanamicin; Ak, amikacin; Gm, gentamicin; Nal, nalidixic acid; Ri, rifampin; Su, sulfamide; Tc, tetracycline; Hg, mercuric ions.

  • b Gm phenotype conferred by AAC(6′)-Ib9 with Ser at position 119.