Table 1.

Characteristics of the bacterial strains and plasmids used in this study

E. coli strain or plasmidRelevant characteristicsaSource or reference
Strains
 DH5α deoR supE44 Δ(lacZYA-argFV169) (φ80 dlacZΔM15) Fλ hsdR17(rk mk +) recA1 endA1 gyrA96 thi-1 relA1 26
 JRG582Δ (ampDE) 2 18
 STC172 ampD11E + 10
Plasmids
 pEC1CCmr; ampC ampR from E. cloacae 23
 pNH5Kmr; ampD from E. coli 10
 pBGS19+ Kmr f1 OrilacPOZ 28
 pHUL4DE-PHKmr; ampDE region ofP. aeruginosa PAO1 cloned into pBK-CMVThis study
 pHUL4DE-1Kmr; ampDE + ORF-1 fromP. aeruginosa PAO1 cloned into pBGS19+ This study
 pHUL4DE-2Kmr;ampDE from P. aeruginosa PAO1 cloned into pBGS19+ This study
 pHUL4DKmr;ampD from P. aeruginosa PAO1 cloned into pBGS19+ This study
  • a Cm, chloramphenicol; Km, kanamycin.