Table 1.

Strains used in this study

HostPlasmidPhenotypeRestriction enzyme insert (length [kb])VectorMarkeraNickname
E. cloacae8009pFCX300LAmpA AmpRCrypticCTX
E. cloacae 8009pFCX300MAmpA AmpRCrypticCTX
E. cloacae8009pFCX300SCryptic
E. coli DH10B
E. coliDH10BpFCX310AmpA Sau3AI (2.3)pHSG396CP, CTXTF2-2
E. coliDH10BpFCX320AmpA Sau3AI (2.3)pHSG396CP, CTXTF2-3
E. coli DH10BpFCX300LAmpA AmpRCTXTF-L
E. coli DH10BpFCX300MAmpA AmpRCTXTF-M
E. coli DH10BpFCX301AmpA AmpR EcoRI (10)pHSG396CP, CTXTF-L7
E. coliDH10BpFCX302AmpA AmpR EcoRI (10)pHSG396CP, CTXTF-M4
E. cloacae 199SAmpC
E. cloacae 199SpFCX310AmpA Sau3AI (2.3)pHSG396CP, CTX
E. cloacae199SpFCX320AmpA Sau3AI (2.3)pHSG396CP, CTX
E. cloacae 199AmpC (inducible)
E. cloacae199CAmpC (constitutive)
  • a CTX, cefotaxime; CP, chloramphenicol; Rif, rifampin.