Table 2.

Susceptibilities of E. cloacae 8009 and various transformants to β-lactams

StrainPresence of ampRMIC (μg/ml)
CefuroximeCefotaximeCeftizoximeCefoperazoneCeftazidimeCefoselisCefoxitinMoxalactamAztreonamImipenem
E. cloacae8009+>1001006.25>1003.1312.5>1000.7812.50.78
E. coliDH10B6.250.05≦0.0250.10.2≦0.0253.130.10.10.2
E. coliDH10B/pFCX310>100501.56503.133.133.130.2250.39
E. coliDH10B/pFCX320>100501.561003.136.253.130.2250.2
E. coliDH10B/pFCX300L+500.780.050.780.20.13.130.10.780.2
E. coliDH10B/pFCX300M+>1006.250.26.251.560.786.250.26.250.39
E. coliDH10B/pFCX301+>100250.78253.133.136.250.39250.2
E. coliDH10B/pFCX302+>100251.56503.133.136.250.39250.2
E. cloacae199Sa 3.130.05≦0.0250.20.1≦0.0250.780.05≦0.0250.05
E. cloacae199S/pFCX310>100250.39501.561.560.780.056.250.05
E. cloacae199S/pFCX320>100501.56>1003.136.251.560.112.50.05
E. cloacae199b 6.250.20.10.390.20.05>1000.10.10.39
E. cloacae199Cc >100505025501.56>1003.13250.2
E. coliDH5α3.13≦0.025≦0.025≦0.0250.05≦0.0253.130.1≦0.0250.2
E. coliDH5α/FEC-1>100>1006.25>10012.51003.130.39500.2
E. coliKU3290d +>1002550501001.56>10012.5250.2
  • a β-Lactamase nonproducer.

  • b Inducible β-lactamase producer (original strain).

  • c Constitutive β-lactamase producer.

  • d Transformant producing cloned P. vulgaris β-lactamase.