Table 3.

Survival of M. tuberculosis treated with fluoroquinolones while inside human macrophages

StrainFluoroquinoloneConcn (μg/ml)No. of CFU recoveredaSurvival (% of that on day 0)Growth (% of that on day 5)b
Day 0Day 5
TN913 (gyrA +)None08.3 × 104 1.6 × 106  ++c 100
PD161148 (C8-OMe)23.5 × 103 40.2
45.4 × 103 60.3
PD16073 (C8-H)22.9 × 103 30.2
45.2 × 103 60.3
Ciprofloxacin21.4 × 105 ++8.8
44.9 × 104 593.1
TN1626 (gyrA +)None08.2 × 103 4.6 × 105  ++100
PD161148 (C8-OMe)21.9 × 103 230.4
41.1 × 103 130.2
83.0 × 103 370.7
PD160793 (C8-H)24.0 × 104 ++8.7
41.0 × 103 120.2
81.6 × 103 200.3
Ciprofloxacin26.7 × 104 ++15
42.0 × 104 ++4.3
86.0 × 103 731.3
TN606 (containing D94G-resistant gyrase)None7 × 104 4.1 × 106 ++100
PD161148 (C8-OMe)25.3 × 105 ++13
45.3 × 104 761.3
88.7 × 103 120.2
PD160793 (C8-H)24.0 × 106 ++98
41.9 × 106 ++46
88.0 × 105 ++20
Ciprofloxacin22.0 × 106 ++49
41.6 × 106 ++39
81.0 × 106 ++24
TN565 (containing D94H-resistant gyrase)None01.3 × 105 4.4 × 106 ++100
PD161148 (C8-OMe)24.1 × 105 ++9.3
44.8 × 105 ++11
82.2 × 104 170.5
PD160793 (C8-H)21.5 × 106 ++34
41.5 × 106 ++34
87.5 × 105 ++17
Ciprofloxacin21.2 × 106 ++27
41.57 × 106 ++36
81.84 × 106 ++42
TN1627 (containing D94Y-resistant gyrase)None02.3 × 104 1.3 × 106 ++100
PD161148 (C8-OMe)25.3 × 105 ++41
41.6 × 105 ++12
88.9 × 104 ++6.8
PD160793 (C8-H)27.6 × 105 ++58
46.2 × 105 ++48
85.0 × 105 ++38
Ciprofloxacin24.0 × 105 ++31
49.6 × 105 ++74
85.5 × 105 ++42
  • a Values are averages of two determinations. Similar results were obtained in two additional experiments.

  • b Values below 100% indicate that growth was inhibited.

  • c ++, growth occurred but was not quantified, and so survival was not calculated.