Table 3.

Kinetic parameters of the P. aeruginosa metallo-β-lactamase at pH 7 and 30°C

Antibioticskcat(s−1)Km (μM)kcat/Km (μM−1 s−1)
Benzylpenicillin320 ± 30520 ± 300.62
Ampicillin950 ± 50200 ± 254.8
CarbenicillinNDa ND0.02
PiperacillinNDND0.72
TemocillinND>2,000<0.00010
Ticarcillin 1.1  7400.0015
Imipenem46 ± 339 ± 41.2
Meropenem50 ± 510 ± 20.12
Panipenem44 ± 630 ± 31.5
Biapenem160 ± 2028 ± 26
Nitrocefin63 ± 1027 ± 32.3
Cephaloridine53 ± 222 ± 22.4
Cephalothin48 ± 421 ± 22.4
Cefuroxime8 ± 137 ± 30.22
Ceftazidime8 ± 144 ± 30.18
Cefotaxime1.3 ± 0.54 ± 0.5b 0.35
Desacetyl-cefotaximeNDND0.72
Cefpirome9 ± 114 ± 3b 0.64
Cefepime7 ± 0.511 ± 1b 0.66
Cefoxitin16 ± 18 ± 1b 2
Moxalactam88 ± 1010 ± 2b 8.8
LoracarbefNDND0.9
Aztreonam>0.01>1,000<0.0001
Carumonam>0.01>1,000<0.0001
  • a ND, not determined.

  • b Km was obtained as theKi value.